GSM3523709: Rpb3_Nocodazole_0h_Input_B; Saccharomyces cerevisiae; ChI... - SRA

SRX5170657: GSM3523709: Rpb3_Nocodazole_0h_Input_B; Saccharomyces cerevisiae; ChIP-Seq
1 ILLUMINA (NextSeq 500) run: 26.2M spots, 2.6G bases, 965.5Mb downloads

Submitted by: NCBI (GEO)

Study: Contrasting roles of the RSC and ISW1/CHD1 chromatin remodelers in RNA polymerase II elongation and termination

PRJNA296949 • SRP066979 • All experiments • All runs

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We addressed the roles of four remodeling machines (ISW1, ISW2, CHD1 and RSC) in specifying the chromatin organization. Overall design: Comparison of budding yeast nucleosome positions obtained using micrococcal nuclease digestion followed by paired-end sequencing (MNase-seq). We sequenced nucleosomes from isogenic strains with the essential RSC8 gene linked to a GAL promoter and isw1?, isw2? or chd1? mutations in all combinations. We measured gene activity by ChIP-seq for the Rpb3 subunit of RNA polymerase II.

Sample: Rpb3_Nocodazole_0h_Input_B

SAMN10620655 • SRS4178292 • All experiments • All runs

Organism: Saccharomyces cerevisiae

Library:

Instrument: NextSeq 500

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: PAIRED

Construction protocol: ChIP-seq experiments: cells were formaldehyde cross-linked before harvesting (1% for 15 minutes). The IP was performed with anti-Rpb3 antibody (Neoclone WP012) The DNA was repaired using the DNA repair kit from New England Biolabs. The repaired DNA was processed for paired-end sequencing according to the Illumina protocol.

Experiment attributes:

GEO Accession: GSM3523709

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 26.2M spots, 2.6G bases, 965.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR8359965	26,185,258	2.6G	965.5Mb	2019-01-20

ID:: 6985276

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